Thursday, August 30, 2012

Lactic Acid Bacteria (cocci) Identification Test

Lactic acid bacteria

Lactococcus lactis, Lactococcus lactis subsp. lactis biovar diacetylactis, Lactococcus lactis subsp. cremoris, Streptococcus thermophilus, Pedicoccus acidilactici and Leuconostoc sp.

Morphology: Cocci
Gram: Positive
Catalase: Negative
Respiratory system: Facultative anaerobe


Tests to identify the genus

  • Growth at 10° C and 45° C - Elliker/M17 broth
  • NaCl resistance: 2, 4, 6 % NaCl
  • CO2 production - MRS medium with bell

Tests to identify the species

  • Acidification and reduction test
  • Citratase test
  • Arginine dihydrolase test - Moeller medium
  • Sugar fermentations: glucose, sucrose, ribose, xylose, maltose, lactose
Please, feel free to comment if there is any mistake.

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