Thursday, August 30, 2012

Lactic Acid Bacteria (rods) Identification Test

Lactic acid bacteria (rods)

Lactobacillus casei, L. plantarum, L. fermentum, L. acidophilus, L. bulgaricus and L. helveticus

Morphology: Rods
Gram: Positive
Catalase: Negative
Motility: Non-motile
Respiratory system: Facultative aerobe



Tests to identify the genus Lactobacillus 

  • CO2 production from glucose
  • Culture at 15° C and 45° C - MRS medium
  • Arginine dihydrolase - Moeller medium
  • Sugar fermentation : ribose, lacotse, maltose, sorbitol or sucrose

Test to identify the species

  • Esculin hydrolysis - Esculin agar
  • Acidification and reduction

Please, feel free to comment if there is any mistake.

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