Eterobacteriaceae Identification Test

Enterobacteriaceae

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Morphology: Rod-shape
Gram: Negative
Oxidase: Negative
Motility: Peritrichous (except Klebsiella sp. & Shigella sp.)
Respiratory system: Facultative anaerobe

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Tests to identify genus & species

Carbon metabolism

• Methyl red & Voges Proskauer - Clark & Lubs medium
• Glucose and lactose fermentation, gas and H₂S production - Kligler medium
• β-galactosidase - ONPG
• Citrate utilization - Simmons medium
• Malonate utilization - Leifson medium

Nitrogen metabolism

• Gelatinase test
• Amino-acids deamination
• Lysine decarboxylase - Taylor or Moeller media with and without Lysine
• Indole production - Peptone water indole-free
• Urease - Christensen medium

IMViC - Indole Methyl red Voges Proskauer Citrate

Micro-organism	Indole	MR	VP	Citrate
E. coli	+	+	-	-
Edwardsiella	+	+	-	-
Proteus sp.	+	+	-	-
Klebsiella pneumoniae	-	+	-	+
Klebsiella oxytoca	+	-	+	+
Enterobacter sp.	-	-	+	+
Serratia sp.	-	-	+	+
Citrobacter freundii	-	+	-	+
Citrobacter koseri	+	+	-	+

Please, feel free to comment if there is any mistake.