Clostridium sp. Identification Test

Clostridium sp.

Morphology: Rods

Gram: Positive

Catalase: Negative
Oxidase: Negative

Respiratory system: Obligate anaerobe

Motility: Motile

Characteristic: Produces endospores

Tests to identify the genus

API 20A
Rosenow medium
Nitrate reductase test
Proteolysis and acidification
Sulfites reduction
Lecithinase test

Test to identify butyric Clostridium

Lactate utilization: butyric acid and CO₂ production - Bryant and Burkey medium

Please, feel free to comment if there is any mistake

Lactic Acid Bacteria (rods) Identification Test

Lactic acid bacteria (rods)

Lactobacillus casei, L. plantarum, L. fermentum, L. acidophilus, L. bulgaricus and L. helveticus

Morphology: Rods

Gram: Positive

Catalase: Negative
Motility: Non-motile
Respiratory system: Facultative aerobe

Tests to identify the genus Lactobacillus

CO₂ production from glucose
Culture at 15° C and 45° C - MRS medium
Arginine dihydrolase - Moeller medium
Sugar fermentation : ribose, lacotse, maltose, sorbitol or sucrose

Test to identify the species

Esculin hydrolysis - Esculin agar
Acidification and reduction

Please, feel free to comment if there is any mistake.

Eterobacteriaceae Identification Test

Enterobacteriaceae

Morphology: Rod-shape
Gram: Negative
Oxidase: Negative
Motility: Peritrichous (except Klebsiella sp. & Shigella sp.)
Respiratory system: Facultative anaerobe

Tests to identify genus & species

Carbon metabolism

Methyl red & Voges Proskauer - Clark & Lubs medium
Glucose and lactose fermentation, gas and H₂S production - Kligler medium
β-galactosidase - ONPG
Citrate utilization - Simmons medium
Malonate utilization - Leifson medium

Nitrogen metabolism

Gelatinase test
Amino-acids deamination
Lysine decarboxylase - Taylor or Moeller media with and without Lysine
Indole production - Peptone water indole-free
Urease - Christensen medium

IMViC - Indole Methyl red Voges Proskauer Citrate

Micro-organism	Indole	MR	VP	Citrate
E. coli	+	+	-	-
Edwardsiella	+	+	-	-
Proteus sp.	+	+	-	-
Klebsiella pneumoniae	-	+	-	+
Klebsiella oxytoca	+	-	+	+
Enterobacter sp.	-	-	+	+
Serratia sp.	-	-	+	+
Citrobacter freundii	-	+	-	+
Citrobacter koseri	+	+	-	+