Bacillus sp. Identification Test

Bacillus sp.

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Morphologie: Rods

Gram: Positive

Catalase: Positive

Respiratory system: Facultative aerobe (B. subtilis: Obligate aerobe)

Motility: Peritrichous
Characteristic: Produces endospores

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Tests to identify spieces

• CO₂ production - Gibson Abdel Malek (GAM) medium
• Acetyl-methyl-carbinol (AMC) production - AMC medium
• Indole production - Peptone water indole-free
• Urease test - Christensen medium
• Citrate utilization - Simmons medium
• Growth at 65° C
• 7 % NaCl resistance
• Proteolysis test
• Gelatinase test
• Amylase test
• Hemolysis - Sheep blood agar
• Sugar fermentations: glucose, arabinose, mannitol and xylose

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Clostridium sp. Identification Test

Clostridium sp.

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Morphology: Rods

Gram: Positive

Catalase: Negative
Oxidase: Negative

Respiratory system: Obligate anaerobe

Motility: Motile

Characteristic: Produces endospores

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Tests to identify the genus

• API 20A
• Rosenow medium
• Nitrate reductase test
• Proteolysis and acidification
• Sulfites reduction
• Lecithinase test

Test to identify butyric Clostridium

• Lactate utilization: butyric acid and CO₂ production - Bryant and Burkey medium

Please, feel free to comment if there is any mistake