Lactic Acid Bacteria (rods) Identification Test

Lactic acid bacteria (rods)

Lactobacillus casei, L. plantarum, L. fermentum, L. acidophilus, L. bulgaricus and L. helveticus

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Morphology: Rods

Gram: Positive

Catalase: Negative
Motility: Non-motile
Respiratory system: Facultative aerobe

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Tests to identify the genus Lactobacillus 

• CO₂ production from glucose
• Culture at 15° C and 45° C - MRS medium
• Arginine dihydrolase - Moeller medium
• Sugar fermentation : ribose, lacotse, maltose, sorbitol or sucrose

Test to identify the species

• Esculin hydrolysis - Esculin agar
• Acidification and reduction

Please, feel free to comment if there is any mistake.

Lactic Acid Bacteria (cocci) Identification Test

Lactic acid bacteria

Lactococcus lactis, Lactococcus lactis subsp. lactis biovar diacetylactis, Lactococcus lactis subsp. cremoris, Streptococcus thermophilus, Pedicoccus acidilactici and Leuconostoc sp.

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Morphology: Cocci

Gram: Positive

Catalase: Negative
Respiratory system: Facultative anaerobe

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Tests to identify the genus

• Growth at 10° C and 45° C - Elliker/M17 broth
• NaCl resistance: 2, 4, 6 % NaCl
• CO2 production - MRS medium with bell

Tests to identify the species

• Acidification and reduction test
• Citratase test
• Arginine dihydrolase test - Moeller medium
• Sugar fermentations: glucose, sucrose, ribose, xylose, maltose, lactose

Please, feel free to comment if there is any mistake.