Lactic Acid Bacteria (rods) Identification Test

Lactic acid bacteria (rods)

Lactobacillus casei, L. plantarum, L. fermentum, L. acidophilus, L. bulgaricus and L. helveticus

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Morphology: Rods

Gram: Positive

Catalase: Negative
Motility: Non-motile
Respiratory system: Facultative aerobe

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Tests to identify the genus Lactobacillus 

• CO₂ production from glucose
• Culture at 15° C and 45° C - MRS medium
• Arginine dihydrolase - Moeller medium
• Sugar fermentation : ribose, lacotse, maltose, sorbitol or sucrose

Test to identify the species

• Esculin hydrolysis - Esculin agar
• Acidification and reduction

Please, feel free to comment if there is any mistake.